山羊子宫内膜细胞与性腺激素对uNK细胞分泌活性的调节作用

doi:10.11843/j.issn.0366-6964.2013.06.006

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (6): 866-870.doi: 10.11843/j.issn.0366-6964.2013.06.006

山羊子宫内膜细胞与性腺激素对uNK细胞分泌活性的调节作用

廖庆红，丁培阳，赵丹丹，王爱华，齐雪峰^*

（西北农林科技大学动物医学院，杨凌 712100）

收稿日期:2013-01-05 出版日期:2013-06-23 发布日期:2013-06-23
通讯作者: 齐雪峰，副教授，博士，E-mail：yxyan2002@126.com
作者简介:廖庆红(1989-)，女，广西桂林人，本科生，主要从事家畜内分泌学与免疫学相关研究，E-mail: liaoqh729@163.com
基金资助:
国家自然科学基金（30901089）；国家博士点基金资助项目（20090204120011）；中央高校基本科研业务费专项重点项目(ZD2013008)

Regulatory Role of Goat Endometrial Cells and Sex Gland Hormones in uNK Cells Secretion

LIAO Qing-hong, DING Pei-yang, ZHAO Dan-dan, WANG Ai-hua, QI Xue-feng^*

（College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China）

Received:2013-01-05 Online:2013-06-23 Published:2013-06-23

摘要/Abstract

摘要：

为探讨山羊子宫内膜细胞和性腺激素对子宫自然杀伤（uNK）细胞分泌血管内皮生长因子（VEGF）和γ-干扰素（IFN-γ）含量的影响。本研究以永生化山羊子宫内膜基质细胞（ESC）和上皮细胞（EEC）为体外研究模型，通过ELISA法检测子宫内膜细胞和性腺激素（E₂ 和P₄）对uNK细胞分泌VEGF和IFN-γ含量的影响。结果显示，在uNK细胞与EEC共培养时，雌激素E₂单独或与P₄共同作用可显著抑制uNK细胞中IFN-γ和VEGF分泌水平（P<0.05），而P₄单独作用不显著；而当uNK细胞与ESC共培养时，孕酮P₄单独或与E₂共同作用可显著增强IFN-γ在uNK细胞中的分泌水平（P<0.05），而E₂单独或与P₄共同作用可显著抑制VEGF分泌水平（P<0.05）。研究结果表明性腺激素对与 EEC或ESC共培养的uNK细胞分泌水平具有不同程度的调节作用。

Abstract:

To explore the effects of goat endometrial cells and sex hormones on secretion content of VEGF and IFN-γ in uNK cells. Using immortalized goat endometrial stromal cells (ESC) and epithelial cells (EEC) as an in vitro research model, the effects of the endometrial cells and sex hormones (E₂, P₄) on secretion content of VEGF and IFN-γ in uNK cells was detected by ELISA assay. When uNK cells co-cultured with EEC, estrogen alone or together with P₄ significantly inhibited the secretion levels of IFN-γ and VEGF in uNK cells (P<0.05), while the P₄ alone had no significant effect. When uNK cells co-cultured with ESC, P₄ alone or together with E₂ can significantly enhance the secretion of IFN-γ in uNK cells (P<0.05), whereas E₂ alone or together with P₄ significantly inhibited the secretion level of VEGF (P<0.05). These results indicated that the sex hormones play a role in regulatoring secretion of uNK cells co-cultured with EEC or ESC.

中图分类号:

S827
Q813.1

廖庆红，丁培阳，赵丹丹，王爱华，齐雪峰. 山羊子宫内膜细胞与性腺激素对uNK细胞分泌活性的调节作用[J]. 畜牧兽医学报, 2013, 44(6): 866-870.

LIAO Qing-hong, DING Pei-yang, ZHAO Dan-dan, WANG Ai-hua, QI Xue-feng. Regulatory Role of Goat Endometrial Cells and Sex Gland Hormones in uNK Cells Secretion[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 866-870.

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参考文献

ROBERTS R M, CHEN Y, EZASHI T, et al. Interferons and the maternal-conceptus dialog in mammals ［J］. Semi Cell Dev Biol, 2008, 19(2): 170-177.

LEANN B, KAZUYOSHI H, CHRISTOPH V. Blastocyst elongation, trophoblastic differentiation, and embryonic pattern formation ［J］. Reproduction, 2008, 135(2): 181-195.

DIMITRIADIS E, WHITE C A, JONES R L, et al. Cytokines, chemokines and growth factors in endometrium related to implantation［J］. Hum Reprod Update, 2005, 11(6): 613-630.

SANTONI A, ZINGONI A, CERBONI C, et al. Natural killer (NK) cells from killers to regulators:Distinct features between peripheral blood and decidual NK cells［J］. Am J Reprod Immunol, 2007, 58 (3): 280-288.

OH M J, CRO B A. A map of relationships between uterine natural killer cells and progesterone receptor expressing cells during mouse pregnancy ［J］. Placenta, 2008, 29(4): 317-323.

BIZINOTTO M C, TAMASHIRO W M, GABRIEL D L, et a1. Uterine natural killer cells are immunogenic in syngeneic male mice ［J］. J Reprod Immunol, 2008, 79(1):18-25.

PFARRER C, RAZIWA S, WINTHER H, et al. Localizagtion of vascular endothelial growth factor (VEGF) and its receptors VEGFR-1 and VEGFR-2 in bovine placentomes from implantation until term ［J］. Placenta, 2006,27(8):889-898.

MANASTER I, MANDELBOIM O. The unique properties of human NK cells in the uterine mucosa ［J］. Placenta, 2008, 29 （supplement A）: S60-S66.

SAITO S, NAKASHIMA A, MYOJO-HIGUMA S, et a1. The balance between cytotoxic NK cells and regulatory NK cells in human pregnancy ［J］. J Reprod Immunol, 2008,77(2): 14-22.

HERINGTON J L, BANY B M. Effect of the conceptus on uterine natural killer cell numbers and function in the mouse uterus during decidualization［J］. Biol Reprod, 2007,76(4): 579-588.

盛红霞，吴庆侠，靳亚平，等. 山羊子宫内膜基质细胞永生化研究［J］.畜牧兽医学报, 2009，40(6): 818-823.

吴庆侠, 王爱华, 司永嘉, 等. 山羊子宫内膜上皮细胞转染pCI-neo-hTERT质粒后的永生化［J］.中国兽医学报, 2010, 30(2):228-232.

WANG H B, LU S H, LIN Q X, et al. Reconstruction of endometrium in vitro via rabbit uterine endometrial cells expanded by sex steroid［J］. Fertil Steril, 2010, 93(7):2385-2395.

陈秀荔, 靳亚平, 张彦明. 分离培养兔子宫内膜细胞的鉴定及性激素对子宫内膜间质细胞形态的影响［J］.畜牧兽医学报, 2006, 37(11): 1226-1231.

LASH G E, SCHIESS I B, KIRKLEY M, et al. Expression of angiogenic growth factors by uterine natural killer cells during early pregnancy ［J］. J Leu Biol, 2006, 80(3):572-580.

ASHKR A A, DISANTO J P, CROY B A. Interferon γ contributes to initiation of uterine vascular modification, decidual integrity, and uterine natural killer cell maturation during normal murine pregnancy ［J］. J Exp Med, 2000, 192(2): 259-270.

TSUJI Y, TAMAOKI T H, HASEGAWA A,et al. Expression of interleukin-18 and its receptor in mouse ovary［J］. Am J Repord Immunol,2001,46:349-357.

LEDEE N, CHAOUAT G, SERAZIN V, et al. Endometrial vascularity by three-dimensional power Doppler ultrasound and cytokines: a complementary approach to assess uterine receptivity［J］. J Reprod Immunol, 2008, 77(1): 57-62.

QI X F, NAN Z C, QU Y Y, et al. Stromal-epithelial interactions modulate the effect of ovarian steroids on goat uterine epithelial cell interleukin-18 release［J］. Domest Anim Endocrin, 2012, 42(4): 210-219.

屈延延，齐雪峰，南志春，等.性腺激素对山羊子宫内膜上皮细胞激素受体表达的影响［J］. 畜牧兽医学报，2013 ，44(1): 135-139.

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山羊子宫内膜细胞与性腺激素对uNK细胞分泌活性的调节作用

Regulatory Role of Goat Endometrial Cells and Sex Gland Hormones in uNK Cells Secretion

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